Lymphoprep 50 ml
isolate mononuclear cells from peripheral blood, cord blood, or bone marrow with Lymphoprep™—a cost-effective alternative to Ficoll-Paque™.
Use this density gradient medium for rapid, simple, and reliable cell isolation from most blood samples obtained from normal individuals and patients. You can substitute Lymphoprep™ for Ficoll-Paque™ without changing your existing protocols and achieve similar cell purity and recovery rates.
This medium is fully compatible with both SepMate™ and RosetteSep™. Formulated with sodium diatrizoate (9.1% w/v) and polysaccharide (5.7% w/v), Lymphoprep™ has a density of 1.077 g/ml.CONTAINS• Sodium diatrizoate (9.1% w/v)
• Polysaccharide (5.7% w/v)
• Other ingredientsSUBTYPEDensity Gradient MediaCELL TYPEMononuclear CellsSPECIESHumanSAMPLE SOURCEBone Marrow, Cord Blood, Whole BloodSELECTION METHODNegativeAPPLICATIONCell IsolationBRANDLymphoprep
Lymphoprep™ Isolation of human mononuclear cells
A simple and effective method for the isolation of mononuclear cells from human blood was reported by Dr. Arne Bøyum in 1968. For more than 35 years a commercial medium known as Lymphoprep™ has been widely used for isolating these cells.
Mononuclear cells (monocytes and lymphocytes) have a lower buoyant density than the erythrocytes and the polymorphonuclear (PMN) leukocytes (granulocytes). The vast majority of mononuclear cells have densities below 1.077 g/ml. These cells can therefore be isolated by centrifugation on an isoosmotic medium with a density of 1.077 g/ml, which allows the erythrocytes and the PMNs to sediment through the medium while retaining the mononuclear cells at the sample/medium interface.
Lymphoprep™ is a ready-made, sterile and endotoxin tested solution with the following specifications:
- Sodium diatrizoate: 9.1% (w/v)
- Polysaccharide: 5.7% (w/v)
- Density: 1.077 ± 0.001 g/ml
- Osmolality: 290 ± 15
Endotoxins: < 1.0 EU/ml